The Unified Flow Core provides state-of-the-art flow cytometry and cell sorting services to the entire University of Pittsburgh research community as well as researchers at neighboring institutions. A skilled staff provides help with instrument setup, data analysis, and consultation for experiment design. Training is available to enable investigators and their staff to run the analytical cytometers themselves.
For information about the ITD core services, or to initiate a CRISPR project, see the core services, or contact Dr. Sebastien Gingras, ITD Core Director, at sgingras@pitt.edu.
The Mouse Embryo Services (MES) and Innovative Technologies Development (ITD) Cores are a shared resource designed to produce genetically engineered mice for investigators at the University of Pittsburgh. Our laboratory provides a broad, comprehensive range of services to investigators.
For information about the MES core services, or to initiate a project, visit our page or contact Dr. Chunming Bi, Core director, at chunming@pitt.edu.
Epoch™ has a monochromator-base UV-Vis wavelength selection. Wavelength range from 200 nm to 999 nm and 6- to 384- microplate reading capability, controlled with the Gen5 Data Analysis software interface. In addition, micro-volume quantification is fast and easy with the Take3 Micro-Volume plates. Pre-programmed nucleic acid and protein protocols in Gen5 make quantification fast and easy. For more information, please contact Pamela Beatty, at 412-383-5287.
ChemiDoc Imagers offer best-in-class performance with ease of use for visible light (RGB) and far red/near infrared (FR/NIR) fluorescence and chemiluminescence detection and all general gel documentation applications. Stain-free imaging enables immediate visualization of proteins without gel staining and instant verification of protein transfer to blots. Features of the ChemiDoc MP include Chemiluminescence sensitivity matching X-ray film and multiplex fluorescence defection. For more information, please contact Rachel Gottschalk
- Immune-mediated disease diagnostics:
- Autoantibodies (screening and identification) (serum)
- Connective tissue diseases (RA, SLE, SS, sSS, …)
- Autoimmune GI diseases (liver, Celiac, …)
- Thyroiditis
- ANCA vasculitis
- Multiple sclerosis, isoelectrofocusing, BBB integrity (serum, CSF)
- Infection disease antibodies and antigens (HIV, HSV, EBV, HBV, HCV, VZV, Rubella, Mumps, measles, COVID-19, …)
- Allergen-specific IgE (serum)
- Total immunoglobulins and subclasses (serum), C3, C4 (plasma)
- Total protein electrophoresis with immunofixation (serum, urine)
- Autoantibodies (screening and identification) (serum)
- Remnant serum samples for specific diseases (IRB approval) For questions, requests, method validation, and quotes, please contact Michael Shurin and Sarah Wheeler
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