Please click on the instrument name for more information about its current configuration. You can schedule time on any instrument you have been trained to use via iLab. To schedule a sort on any of the sorters listed below, please use iLab as well, understanding that your proposed sort time is not valid until it has been approved by UFC staff, usually within one business day. (Sorts not yet approved appear in orange; approved sorts appear in purple.) Please refer to Core policies on Scheduling and Cancelation.
When planning new experiments, we recommend using the panel builder app at FluoroFinder (free registration for Pitt users).
If you experience any issues with a machine after hours or on a weekend, please use the Problem Log to report it, and we will resolve the issue as soon as we can.
| Instrument Name | Type | Location | Name in FluoroFinder |
|---|---|---|---|
| Aria Assembly Hood (was Aria 10 Hood) | Traditional Flow Sorter | Ford - G536 | Aria Assembly G536 |
| Attune | Traditional Flow Analyzer | Ford - G530 | Attune NxT Assembly G530 |
| Aurora Blue | Spectral Flow Analyzer | Ford - G530 | Aurora Blue Assembly G530 |
| Aurora Pink | Spectral Flow Analyzer | Ford - G530 | (same config as Aurora Blue) |
| Fortessa Assembly (was Fortessa 15) | Traditional Flow Analyzer | Ford - G530 | Fortessa Assembly G530 |
| ImageStream | Imaging Flow Analyzer | Ford - G536 | ImageStream Mark II Assembly G536 |
| LSR Assembly (was LSR 10) | Traditional Flow Analzer | Ford - G530 | LSR II Assembly G530 |
| ZellScanner | Chip Cytometer | Ford - G536 | N/A |
| Aria-Assembly 5 Laser (replaced Aria 3 laser) | Traditional Flow Sorter | BST - E1514 | Aria 5 Laser | Assembly G536 |
| Aria-15 Hood 5 Laser (was Aria 15 Hood) | Traditional Flow Sorter | BST - E1514 | Aria 5 Laser BST E1514 |
| Aurora Black | Spectral Flow Analyzer | BST - E1514 | Aurora Black BST E1514 |
| Aurora Yellow | Spectral Flow Analyzer | BST - E1514 | Aurora Yellow BST E1514 |
| Fortessa 7S (Hood) | Traditional Flow Analyzer | BST - S756 | Fortessa BST S756 |
| Fortessa 12 | Traditional Flow Analyzer | BST - E1211 | Fortessa BST E1211 |
| LSR 15 | Traditional Flow Analyzer | BST - E1514 | LSR II BST E1514 |
| Aurora CS | Spectral Flow Sorter | BST - E1514 | Aurora CS BST E1514 |
- AURORA
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The Auroras are equipped with 5 lasers - 488 (blue), 640 (red), 405 (violet), 561 (yellow-green) 355 (UV).
64 Fluorescence channels: 355nm = 16 channels; 405nm = 16 channels; 488nm = 14 channels; 640nm = 8 channels; 561nm = 10 channels. Efficient spectrum capture for dyes emitting in the 360nm-900nm range. No filter changes required for any fluorochromes.
Aurora Black has a plate loader for 96 well plates.
Aurora Pink and Aurora Yellow have a plate loader for 96 well plates and a 40 tube holder.
- Aurora CS
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Aurora CS (Cell Sorter)
The Aurora CS is equipped with 5 lasers - 488 (blue), 640 (red), 405 (violet), 561 (yellow-green) 355 (UV).
64 Fluorescence channels: 355nm = 16 channels; 405nm = 16 channels; 488nm = 14 channels; 640nm = 8 channels; 561nm = 10 channels. Efficient spectrum capture for dyes emitting in the 360nm-900nm range. No filter changes required for any fluorochromes.
6 way sorts into 1.5 ml Eppendorf tubes. 4 way sorts into 5ml FACS tubes. 2 way sorts into 15ml Falcon tubes. Single Cell sorting into 96 well plates (no other plate sizes at this time)
- Cytek web page
- Aurora tested fluorochromes
- Aurora filter range (same as Aurora analyzers)
- IMAGESTREAM
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Lasers: 488nm 200mW - 561nm 200mW - 375nm 70mW - 405nm 175mW - 642nm 150mW - 785nm Dark Field Laser
MultiMag 60X,40X,20X
Extended Depth of Field (EDF)
Auto-Sampler (96 well plates)
12 Imaging Channels
Detector name Band (nm) Fluorochromes Channel 1 435-480 475/45 DAPI, BV421, PacBlue Channel 2 480-560 428/65 BV510, PacOrange -- FITC, GFP Channel 3 569-595 528/65 QD565 -- Cy3, DSRed -- PE Channel 4 595-642 610/30 QD625 -- PE-TXRed, RFP -- mCherry Channel 5 642-745 702/85 QD705, BV711 -- PerCP -- PeCy5, DRAQ5 Channel 6 745-780 762/35 QD800,BV786 -- PeCy7 --Pe-AF750 Channel 7 435-505 457/45 DAPI, BV 421 Channel 8 505-570 537/65 BV510, QD525 Channel 9 570-595 582/25 QD565,QD585 -- Bright Field Channel10 595-642 610/30 QD625,BV605 Channel 11 642-745 702/85 QD705,BV711 -- APC, AF700 Channel 12 745-780 762/35 BV786 -- APC-Cy7 -- AF750 - ARIA Assembly 5 LASER
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The Aria is equipped with 5 lasers 488 (blue), 640 (red), 405 (violet), 552 (light green) and 355 (UV) for a total of 16 fluorescent parameters running on Diva 9/Windows 10.
Detector name LP Mirror BP Filter Fluorochromes 355 Laser A 690 740/35, BUV737 355 Laser B --- 379/28 BUV395 405 Laser A 750 780/60 BV786 405 Laser B 690 710/50 BV711 405 Laser C 630 660/20 BV650, QD655 405 Laser D 505 525/50 AmCyan, VioGreen, PacGreen, BV510 405 Laser E --- 450/50 PacBlue, V450, BV421, VioBlue, eBFP 488 Laser A 685 710/50 PerCP-Cy5.5 488 Laser B 505 530/30 FITC, GFP, AF488 552 Laser A 750 780/60 PE-Cy7 552 Laser B 685 710/50 PE-Cy5.5 552 Laser C 600 610/20 PE, TdTomato 552 Laser D --- 575/26 PE-TxRed, mCherry, 7AAD 635 Laser A 750 780/60 APC-Cy7, APC-Fire750 635 Laser B 690 730/45 AF700 635 Laser C --- 670/30 APC, AF647, Cy5 - FORTESSA ASSEMBLY (was Fortessa 15)
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The FORTESSA is equipped with 5 lasers 488 (blue), 640 (red), 405 (violet), 532 (Green), 355 (UV) for a total of 16 fluorescent parameters, running on Diva 8/Windows 7. Lasers: 405 100mW Trigon, 488 50mW Octagon, 532 150mW Octagon, 640 100mW Trigon, 355 20mW Trigon.
Detector name LP Mirror BP Filter Fluorochromes 532 Laser A 750 780/60 PE-CY7 532 Laser B 670 695/40 PE-CY5.5 532 Laser C 635 670/30 PE-Cy5 532 Laser D 600 610/20 PE-TxRED,ECD 532 Laser E --- 575/26 PE 640 Laser A 785 780/60 APC-Cy7 640 Laser B 735 720/20 Alexa 700 640 Laser C --- 660/20 APC 405 Laser A 595 610/20 BV605 405 Laser B 505 525/50 V500 405 Laser C --- 450/50 Pacific Blue 488 Laser A 685 710/50 PerCP Cy5.5 488 Laser B 655 670/30 PerCP 488 Laser C 505 530/30 (515/20) FITC (GFP) 488 Laser D --- 488/10 SSC 355 Laser A 690 740/35 BUV 737 505 530/30 Hoechst 355 Laser B --- 379/28 BUV 395 450/50 DAPI, Alexa 350 - LSR ASSEMBLY (was LSR 10)
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The LSR II is equipped with 5 lasers running on Diva 8/Windows 7. Lasers: 355 20mW Trigon (UV), 405 50mW Trigon (violet), 488 20mW Trigon (blue), 532 150mW Octagon (green), 640 100mW Trigon (red). 15 parameters.
Detector name LP Mirror BP Filter Fluorochromes 488 Laser A 635 695/40 PerCP/PerCP-Cy5.5 488 Laser B 505 525/50 FITC, GFP 488 Laser C --- 488/10 SSC 532 Laser A 705 780/60 PE-Cy7 532 Laser B 685 710/50 PE-C5.5 532 Laser C 635 670/30 PE-Cy5 532 Laser D 595 610/20 PETxRED/PI 532 Laser E --- 575/20 PE 640 Laser A 755 780/60 APC-Cy7 640 Laser B 700 720/20 Alexa 700 640 Laser C --- 660/20 APC 405 Laser A 595 610/20 BV605 405 Laser B 505 525/50 V500, BV510, Alexa 430 405 Laser C --- 450/50 Pacific Blue, Alexa 405, BV421,V450 355 Laser A 690 740/35 BUV 737 505 530/30 Indo 1 670LP Red Side pop. 355 Laser B --- 379/28 BUV 395 405/20 Indo-1 High 450/50 DAPI, Alexa 350, Indo Violet - LSR-15
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The LSR II is equipped with 5 lasers running on Diva 8/Windows 7. Lasers: 355 60mW Trigon (UV), 405 50mW Trigon (violet), 488 20mW Trigon (blue), 532 150mW Octagon (green), 633 40mW Trigon (red). 15 Parameters.
Detector name LP Mirror BP Filter Fluorochromes 488 Laser A 635 695/40 PerCP/PerCP-Cy5.5 488 Laser B 505 515/20 FITC/GFP 488 Laser C --- 488/10 SSC 532 Laser A 755 780/60 PE-Cy7 532 Laser B 685 710/50 PE-Cy5.5 532 Laser C 635 670/30 PE-Cy5 532 Laser D 595 610/20 PETxRED/PI 532 Laser E --- 575/20 PE 640 Laser A 755 780/60 APC-Cy7, APC-H7 640 Laser B 700 720/20 Alexa 700 640 Laser C --- 660/20 APC 405 Laser A 595 610/20 BV605 405 Laser B 505 525/50 V500, BV510 405 Laser C --- 450/50 Pacific Blue, BV421,V450 355 Laser A 690 740/35 BUV 737 505 530/30 Indo-1, Hoechst 355 Laser B --- 379/28 BUV 395 405/20 Indo-1 High 450/50 DAPI, Indo-1 low - FORTESSA-7S
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The FORTESSA is equipped with 4 lasers 488 (blue), 640 (red), 405 (violet), 561 (Green), for a total of 13 fluorescent parameters, running on Diva 8/Windows 7. Lasers: 405 100mW Trigon, 488 20mW Trigon, 561 100mW Octagon, 640 40mW Trigon.
Detector Name LP Mirror BP Filter Fluorochromes 561 Laser A 750 780/60 PE-CY7 561 Laser B 670 695/40 PE-CY5.5 561 Laser C 635 670/30 PE-Cy5 561 Laser D 600 610/20 PE-TxRED,ECD 561 Laser E --- 582/15 PE 640 Laser A 750 780/60 APC-Cy7 640 Laser B 685 720/20 Alexa 700 640 Laser C --- 670/30 APC 405 Laser A 600 610/20 BV605 405 Laser B 505 525/50 V500 405 Laser C --- 450/50 Pacific Blue 488 Laser A 685 710/50 PerCP Cy5.5 488 Laser B 505 530/30 (515/20) FITC (GFP) 488 Laser C --- 488/10 SSC - ARIA-15 HOOD 5 LASER (was Aria 15 Hood)
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The Aria is equipped with 5 lasers running Diva 8 on Windows 7. Lasers: 355 20mW Trigon, 405 50mW Trigon, 488 100mW Trigon, 532 150mW Octagon, 635 30mW Trigon. 15 Paramaters.
Detector name LP Mirror BP Filter Fluorochromes 488 Laser A 685 695/40 PerCP/PerCP-Cy5.5 488 Laser B 505 520/15 FITC, GFP 488 Laser C --- 488/10 SSC 532 Laser A 740 780/40 PE-Cy7 532 Laser B 690 710/50 PE-C5.5 532 Laser C 640 660/40 PE-Cy5 532 Laser D 600 610/20 PETxRED/PI 532 Laser E --- 575/26 PE 640 Laser A 735 780/60 APC-Cy7 640 Laser B 710 730/45 Alexa 700 640 Laser C --- 660/20 APC 405 Laser A 600 605/40 BV 610 405 Laser B 505 525/50 V500, BV510, Alexa 430 405 Laser C --- 450/50 Pacific Blue, Alexa 405, BV421,V450 355 Laser A 505 530/30 Indo Blue 690 740/35 BUV 670LP Red Side pop. 355 Laser B --- 379/28 BUV 395 405/20 Indo-1 High 450/40 DAPI, Alexa 350, Indo Violet - Fortessa-12
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The VMI Fortessa is equipped with 4 lasers running Diva 8/Windows 7. Lasers: 355 20mW Trigon (UV), 405 100mW Octagon (violet), 488 100mW Octagon (blue), 640 100mW Trigon (red). 15 Parameters.
Detector name LP Mirror BP Filter Fluorochromes 488 Laser A 750 780/60 PE-Cy7 488 Laser B 685 710/50 PerCP-Cy5.5 488 Laser C 600 610/20 PE-TxRED 488 Laser D 550 575/25 PE 488 Laser E 505 530/30 FITC 488 Laser F --- 488/10 SSC 640 Laser A 750 780/60 APC-Cy7 640 Laser B 690 730/45 Alexa 700 640 Laser C --- 670/30 APC 405 Laser A 630 660/20 Qdot655 405 Laser B 595 605/40 Qdot605
405 Laser C 505 525/50 AmCyan
405 Laser D --- 450/50 Pacific Blue 670LP Red Side pop. 355 Laser A 690 740/37 BUV737
355 Laser B 379/28 BUV395 450/50 DAPI - ARIA ASSEMBLY HOOD (was Aria 10 Hood)
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The Rheum Aria is equipped with 5 lasers running Diva 8/Windows 7. Lasers: 355 60mW Trigon (UV), 405 100mW Trigon (Violet), 488 100mW Trigon (Blue), 532 100mW Octagon (Green), 640 100mW Trigon (Red). 15 Paramaters.
Detector name LP Mirror BP Filter Fluorochromes 532 Laser A 750 780/60 PE-Cy7 532 Laser B 690 710/50 Pe-Cy5.5 532 Laser C 595 670/40 Pe-Cy5 532 Laser D 550 610/20 PE-TxRED 532 Laser E 505 575/25 PE 488 Laser C 690 695/40 PerCP Cy5.5 488 Laser B 505 515/20 FITC 488 Laser A 488 SSC 640 Laser A 750 780/60 APC-Cy7 640 Laser B 690 730/45 Alexa 700 640 Laser C --- 670/30 APC 405 Laser A 570 585/42 BV605 405 Laser B 505 525/50 V500,BV510
405 Laser C --- 450/50 PacBlue, V450,BV421
355 Laser A 690 740/35 BUV737 355 Laser B 379/28 BUV393 - ATTUNE
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The Attune NxT is equipped with 4 lasers 488 (blue), 640 (red), 405 (violet), 561 (Green), for a total of 14 fluorescent parameters, running Windows 7. Lasers: 405 100mW Trigon, 488 100mW Trigon, 561 140mW Octagon, 640 140mW Trigon.
Detector Name Attune Detector Name LP Mirror BP Filter Fluorochromes 532 Laser A GL4 740 780/60 PE-CY7 532 Laser B GL3 650 695/40 PE-CY5.5 532 Laser C GL2 600 620/15 PE-TxRED, ECD 532 Laser D GL1 550 575/36 PE 640 Laser A RL3 740 780/60 APC-Cy7 640 Laser B RL2 690 720/30 Alexa 700 640 Laser C RL1 654 670/14 APC 405 Laser A VL4 650 710/50 BV711 405 Laser B VL3 555 603/48 BV605 405 Laser C VL2 495 512/25 BV510 405 Laser D VL1 417 440/50 BV421 488 Laser A BL3 650 695/40 PerCP, PerCP-Cy5.5 488 Laser B BL2 555 590/40 PE 488 Laser C BL1 503 525/50 FITC - Canopy's ZellScanner One
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Canopy's ZellScanner One is a fully intergrated automated imaging system that enables quantitaitve measure of high-plex protein biomarker assays across a range of sample types. Termed "Chip Cytometry", single cell and whole tissue samples can be loaded onto a chip - the latter via section-on-coverslip attachment. The enlosed chamber acts as a biorepository, providing antibody staining and washing via stain/wash/photobleach cycles, and then stored with tissue preservation for up to two years. This non-destructive analysis allows for multiple interrogations of the same sample.
Filter Sets ZellScanner Filter Set Name "Casual" Name Excititation Emission FS395 BUV395 BP 365/2 nm BP 392/23 nm FS421 DAPI/BV421 BP 390/40 BP 460/50 FS488 FITC/GFP BP 470/40 BP 525/50 FS560 PE BP 545/25 BP 605/70 FSPerCP PerCPCy5.5 BP 435/40 BP 710/75 ALL (Bleach) LP 364